Microglia cells are the immune cells of the central nervous system and consequently play important roles in brain infections and inflammation. Human iPSC-Derived microglia-like cells are generated through embryoid body formation. These protocols mimic the environment and the events of early differentiation of hematopoietic precursors in the developing embryo. In the early stage, they present an amoeboid morphology, but upon cultivation in co-culture with neurons uptake the ramified form. The acquired cells expressed high levels of microglial markers (Iba1, CD11c, TMEM119) but low CX3CR1 and P2YR12, suggesting an early brain developmental stage equivalence. Upon activation, the differentiated and matured human microglia-like cells can produce of various pro-, and antiinflammatory cytokines and presenting with phagocytic activity. In vitro iPSC-based neuronal-astrocyte-microglia co-culture can recapitulate key features of microglial cell activation in AD pathology, presenting a model for disease study and drug screening.

All the cells provided by BioTalentum are negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C. The basic donor information (gender/age) is provided for each cell lot purchased.