Neural stem cells (NSCs) are self-renewing, multipotent cells that generate the main phenotype of the nervous system. They primarily differentiate into neurons, astrocytes, and oligodendrocytes. The recent discovery of induced pluripotent stem cells (iPSCs) not only overcomes the ethical and logistical issues associated with human embryonic stem cells, but also provides a flexible platform for generating various differentiated cell types from diseased individuals. iPSC-derived NSCs are a potentially valuable source of in vitro models for complex, polygenic human diseases, and are potentially useful for drug discovery and cell-based therapy applications. 

BioTalentum provides high quality human neural stem cells (NSCs) derived from normal or diseased iPS cell lines. These cells express typical markers of neural stem and progenitor cells, e.g. Nestin, Pax6 and Sox1, with the purity higher than 90%. The cells have been fully characterized for their self-renewal and multi-potency. The terminally differentiated iPSC-derived NSCs resulted in the formation of neuronal networks and synapses, from the stage of D28, post-synaptic marker PSD95 (now DLG4, Discs Large MAGUK Scaold Protein 4), and the major synaptic vesicle protein p38, Synaptophysin (SYNP) and Vesicle-associated membrane protein 2 (VAMP2) expression appeared in maturing neurons.  Matured synapses can be detected in 42-days-long differentiated samples (Kobolák et al., 2020). 

The hiPSC-based 2D or 3D in vitro neurosphere models could be used effectively to evaluate neurotoxicity, and can be developed further to detect developmental neurotoxicity and thus replace or complement the use of animal models in various basic research and pharmaceutical applications.  

All the cells provided by BioTalentum are negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C. The basic donor information (gender/age) is provided for each cell lot purchased. 

Kobolák et al., Cells 2020, 9(5), 1122; https://doi.org/10.3390/cells9051122