Cellular models for in vitro toxicology
Our hiPSC-based New Approach Models (NAMs) as alternative toxicology models are offered to our customers to replace animal testing systems in preclinical trials. In our test system, terminally differentiated NPC-derived neuronal cultures and astrocytes are used to test customer’s test compounds compared with known neurotoxic agents as positive controls. NPCs are available not only from healthy controls but we offer familiar (f) and sporadic (s) Alzheimer’s disease patient-derived test systems as well.
Our service includes
- Consultation with customers, surveying the needs (no. of tested compounds, no. of dilution, 2D/3D assay required, the differentiation stage of neurons).
- The hiPSC-derived neuron differentiation on a 96-well plate format according to the customer’s requirements. In general, 21 or 42 (TD21,TD42) day differentiated neuronal cultures are used, but the differentiation time can be altered upon request.
- Compound treatment, no. of dilution, no. of biological replicates based on the negotiations with the customer. Paraquat, a well- known neurotoxic agent is used as a positive control.
- Incubation with compounds, for 24, 48 or 72 hours to test the acute and 96 hours to test chronic exposure.
- Viability assay (ATP or resazurin-based) (DB-ALM Protocol No. 207, 208, 209).
- Data analysis to determine the EC50 and IC10 values of the test compound.
- Report for the customer.