Genome editing itself holds tremendous potential for studying genetic causes of a certain diseases, genetic variations. Generation of stable expression systems for in vitro toxicology assays, monitoring molecular events in the response of changes in conditions, environmental effects. We offer customer-specific solutions of required answers with the latest, time and cost-efficient genetic modifications.
We can adapt your choice of cell lines to meet your project requirements. We can provide human iPS cells generated from peripheral blood mononuclear cells. The iPSCs are reprogrammed genetically, by ‘transforming’ genes back into an embryonic-like pluripotent state that allows the development of a virtually unlimited source of any kind human cells needed for the customer’s purposes. This technique has no ethical implications because the cells can be harvested without harming the patients. A wide variety of cells can be created from the same patient and these patient-specific cells can be used to study diseases, toxic effects and for drug testing in vitro.
Several gene-editing techniques allows one to perform scarless editing with a decreased frequency of unspecific events. State-of-the-art techniques improve the efficiency in any custom required endless model generation depending upon the customer’s needs: Knock-in, Knock-out, protein tagging, linking genes, exon modifications and promoter truncation/changes.
Reporter cell lines
Upon request we can generate iPSC-derived in vitro solutions to monitor the required pathway by using fluorescent-tagged reporter genes. This is suitable for drug testing and following stress response. We can help you find the right approach in the initial design to translate to your needs at the genetic level.